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Detection of MCPyV <t>DNA</t> and VP1 and LT mRNAs in <t>FFPE</t> PM specimens and HP tissues. (A) The presence of MCPyV DNA was investigated in PM (n=50) and HP (n=15) FFPEs. (B) The mean MCPyV DNA load was quantified by ddPCR in MCPyV-DNA positive FFPEs (n=16) and reported as mean MCPyV DNA mean viral load copy/cell. (C) MCPyV VP1 and LT mRNA levels, reported as mean 1/ΔCt, were measured by qPCR. B and C panels: Error bars indicate the SEM. (D) No correlations were determined between MCPyV DNA load and VP1 and LT mRNA levels. *, P<0.05. ddPCR, droplet-digital polymerase chain reaction; FFPE, formalin-fixed and paraffin-embedded; HP, healthy pleural; LT, large T; MCPyV, Merkel cell polyomavirus; mRNA, messenger RNA; PM, pleural mesothelioma; SEM, standard error of mean; VP, viral protein; qPCR, quantitative polymerase chain reaction.
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Detection of MCPyV <t>DNA</t> and VP1 and LT mRNAs in <t>FFPE</t> PM specimens and HP tissues. (A) The presence of MCPyV DNA was investigated in PM (n=50) and HP (n=15) FFPEs. (B) The mean MCPyV DNA load was quantified by ddPCR in MCPyV-DNA positive FFPEs (n=16) and reported as mean MCPyV DNA mean viral load copy/cell. (C) MCPyV VP1 and LT mRNA levels, reported as mean 1/ΔCt, were measured by qPCR. B and C panels: Error bars indicate the SEM. (D) No correlations were determined between MCPyV DNA load and VP1 and LT mRNA levels. *, P<0.05. ddPCR, droplet-digital polymerase chain reaction; FFPE, formalin-fixed and paraffin-embedded; HP, healthy pleural; LT, large T; MCPyV, Merkel cell polyomavirus; mRNA, messenger RNA; PM, pleural mesothelioma; SEM, standard error of mean; VP, viral protein; qPCR, quantitative polymerase chain reaction.
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Detection of MCPyV <t>DNA</t> and VP1 and LT mRNAs in <t>FFPE</t> PM specimens and HP tissues. (A) The presence of MCPyV DNA was investigated in PM (n=50) and HP (n=15) FFPEs. (B) The mean MCPyV DNA load was quantified by ddPCR in MCPyV-DNA positive FFPEs (n=16) and reported as mean MCPyV DNA mean viral load copy/cell. (C) MCPyV VP1 and LT mRNA levels, reported as mean 1/ΔCt, were measured by qPCR. B and C panels: Error bars indicate the SEM. (D) No correlations were determined between MCPyV DNA load and VP1 and LT mRNA levels. *, P<0.05. ddPCR, droplet-digital polymerase chain reaction; FFPE, formalin-fixed and paraffin-embedded; HP, healthy pleural; LT, large T; MCPyV, Merkel cell polyomavirus; mRNA, messenger RNA; PM, pleural mesothelioma; SEM, standard error of mean; VP, viral protein; qPCR, quantitative polymerase chain reaction.
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Average 99 stars, based on 1 article reviews
dna extraction - by Bioz Stars, 2026-08
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Detection of MCPyV DNA and VP1 and LT mRNAs in FFPE PM specimens and HP tissues. (A) The presence of MCPyV DNA was investigated in PM (n=50) and HP (n=15) FFPEs. (B) The mean MCPyV DNA load was quantified by ddPCR in MCPyV-DNA positive FFPEs (n=16) and reported as mean MCPyV DNA mean viral load copy/cell. (C) MCPyV VP1 and LT mRNA levels, reported as mean 1/ΔCt, were measured by qPCR. B and C panels: Error bars indicate the SEM. (D) No correlations were determined between MCPyV DNA load and VP1 and LT mRNA levels. *, P<0.05. ddPCR, droplet-digital polymerase chain reaction; FFPE, formalin-fixed and paraffin-embedded; HP, healthy pleural; LT, large T; MCPyV, Merkel cell polyomavirus; mRNA, messenger RNA; PM, pleural mesothelioma; SEM, standard error of mean; VP, viral protein; qPCR, quantitative polymerase chain reaction.

Journal: Translational Lung Cancer Research

Article Title: Pleural mesothelioma patients and ex-exposed asbestos workers are immunologically poor responders to oncogenic Merkel cell polyomavirus

doi: 10.21037/tlcr-2025-198

Figure Lengend Snippet: Detection of MCPyV DNA and VP1 and LT mRNAs in FFPE PM specimens and HP tissues. (A) The presence of MCPyV DNA was investigated in PM (n=50) and HP (n=15) FFPEs. (B) The mean MCPyV DNA load was quantified by ddPCR in MCPyV-DNA positive FFPEs (n=16) and reported as mean MCPyV DNA mean viral load copy/cell. (C) MCPyV VP1 and LT mRNA levels, reported as mean 1/ΔCt, were measured by qPCR. B and C panels: Error bars indicate the SEM. (D) No correlations were determined between MCPyV DNA load and VP1 and LT mRNA levels. *, P<0.05. ddPCR, droplet-digital polymerase chain reaction; FFPE, formalin-fixed and paraffin-embedded; HP, healthy pleural; LT, large T; MCPyV, Merkel cell polyomavirus; mRNA, messenger RNA; PM, pleural mesothelioma; SEM, standard error of mean; VP, viral protein; qPCR, quantitative polymerase chain reaction.

Article Snippet: Total DNA was extracted from FFPEs using the QIAmp DNA FFPE Tissue Extraction Kit (Qiagen, Milan, Italy).

Techniques: Digital PCR, Real-time Polymerase Chain Reaction